Immunotherapeutic targeting of established sarcoma in Swiss mice by tumor-derived antigen-pulsed NLGP matured dendritic cells is CD8+ T-cell dependent
作者： Atanu Mallick , Subhasis Barik , Sarbari Ghosh , Soumyabrata Roy , Koustav Sarkar
DOI: 10.2217/IMT.14.53
关键词:
摘要: Aim: Neem leaf glycoprotein (NLGP) matures human myeloid and mouse bone marrow-derived dendritic cells (DCs). also therapeutically restricts the established sarcoma growth by activating CD8+ T along with increased proportion of tumor residing CD11c+ DCs. Here, we intended to find out whether become cytotoxic after presentation antigen NLGP-matured DCs restrict murine growth. Materials & methods: NLGP was prepared from matured neem(Azadirachta indica) leaves. Solid in Swiss mice developed subcutaneous inoculation cells. GMCSF-IL-4 generated were pulsed for immunotherapy. Status CD8+CD69+T studied flow cytometry secretion cytokines measured ELISA. RT-PCR used monitor status perforin, granzyme B. Results: antigen-pulsed (DCNLGPTAg) inhibit DCNLGPTAg immunization enhanc...
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