Functional electrical properties of the endothelium in lymphatic vessels of the guinea‐pig mesentery

摘要: 1. The resting and agonist-stimulated properties of endothelial cells electrical communication between the endothelium smooth muscle were investigated in open segments guinea-pig mesenteric lymphatic vessels using intracellular microelectrodes. 2. Endothelial had a mean membrane potential (RMP) -71.5 +/- 0.5 mV (n = 100) which was significantly different from value -60.8 1.1 75) recorded muscle. 3. Acetylcholine (ACh, 5-10 microM) generally evoked an initial hyperpolarization followed by depolarization (mean 3.4 15.4 1.0 mV, respectively, n 75). 4. Ca(2+)-activated K+ channels likely to underlie ACh-induced as this response exhibited increased conductance, larger mM solution blocked charybdotoxin (50 nM). 5. did not exhibit nitric oxide (NO) NO-donor sodium nitroprusside alter RMP responses ACh affected NO-synthase inhibitor N omega-nitro L-arginine at concentration markedly inhibited hyperpolarization. 6. Electrical coupling functional there extremely limited continuity (1 12, endothelial/smooth cell simultaneous recordings) bradykinin, noradrenaline isoprenaline caused two types. 7. These results provide first description various agonists its lack with