Non-invasive assessment of fecal progestagens and pregnancy detection in Himalayan musk deer (Moschus chrysogaster).
作者： Chandrasekhar Mithileshwari , Tanushree Srivastava , Vinod Kumar , Ajith Kumar , Govindhaswamy Umapathy
DOI: 10.1016/J.THERIOGENOLOGY.2015.09.009
关键词:
摘要: The Himalayan musk deer (Moschus chrysogaster), an endangered species, is facing threat of extinction globally due to severe hunting for its musk, and efforts are under way in India breed them captivity. However, no information available on the reproductive cycles species. In this study, we aimed standardize enzyme immunoassay (EIA) procedure monitoring pregnancy using fecal samples. We collected samples 12 months from five captive females maintained at Musk Deer Research Centre, Bageshwar, Uttarakhand, India. Three these were observed mating gave birth, whereas two seen but did not give birth. gestation periods three 183, 185, 199 days, respectively. High-pressure liquid chromatography revealed presence immunoreactive pregnanediol-3-glucuronide (PdG), progesterone, 5α-pregnan-3α-ol-20-one (5-alpha-pregnane) metabolites used EIAs against PdG, 5-alpha-pregnane monitor pregnancy. found PdG EIA be a highly accurate sensitive assay compared with other assays detecting conclude that can diagnose steroid analysis, early stage 3 months after mating. This study would help conservation breeding captivity status species wild.
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