Corticotropin releasing factor (CRF) modulates fear-induced alterations in sleep in mice

摘要: Contextual fear significantly reduces rapid eye movement sleep (REM) during post-exposure in mice and rats. Corticotropin releasing factor (CRF) plays a major role CNS responses to stressors. We examined the influence of CRF astressin (AST), non-specific antagonist, on after contextual BALB/c mice. Male were implanted with transmitters for recording via telemetry guide cannula aimed into lateral ventricle. Recordings vehicle handling control obtained ICV microinjection saline (SAL) followed by exposure novel chamber. Afterwards, subjected shock training (20 trials, 0.5 mA, s duration) 2 sessions. After training, separate groups received microinjections SAL (0.2 microl, n=9), (0.4 microg, n=8), or AST (1.0 n=8) prior context alone. Sleep was then recorded 20 h (8-hour light 12-hour dark period). Compared control, decreased REM 8-h period receiving CRF, but not AST. Mice exhibited reductions 12-h fear, whereas did not. also reduced non-REM (NREM) delta (slow wave) amplitude EEG. Only significant NREM total sleep. These findings demonstrate central system regulating alterations induced fear.