Association of MicroRNAs and YRNAs with platelet function

摘要: Rationale: Platelets shed microRNAs (miRNAs). Plasma miRNAs change on platelet inhibition. It is unclear whether plasma miRNA levels correlate with function. Objective: To link small RNAs to reactivity. Methods and Results: Next-generation sequencing of in revealed 2 peaks at 22 23 32 33 nucleotides corresponding YRNAs, respectively. Among predominantly, fragments RNY4 RNY5 were detected. YRNAs measured 125 patients a history acute coronary syndrome who had undergone detailed assessment function 30 days after the event. Using quantitative real-time polymerase chain reactions, 92 assessed different antiplatelet therapies. Key platelet-related correlated tests. MiR-223 ( r p=0.28; n=121; P =0.002), miR-126 p=0.22; =0.016), other abundant showed significant positive correlations vasodilator-stimulated phosphoprotein phosphorylation assay. miR-126, miR-223 also among showing greatest dependency platelets strongly P-selectin, factor 4, basic protein population-based Bruneck study (n=669). A single-nucleotide polymorphism that facilitates processing pri-miR-126 mature accounted for rise circulating activation markers. Inhibition mice reduced aggregation. MiR-126 directly indirectly affects ADAM9 P2Y12 receptor expression. Conclusions: Levels tests markers general population. Alterations affect reactivity. # Novelty Significance {#article-title-46}