The vascular-disrupting agent combretastatin impairs splitting and sprouting forms of physiological angiogenesis.

摘要: Objective: Vascular-disrupting agents like combretastatin (CA-4-P), used to attenuate tumor blood flow in vivo, exert anti-mitotic and anti-migratory effects on endothelial cells vitro. We tested whether anti-vascular or anti-angiogenic of CA-4-P are evident with physiological angiogenesis skeletal muscle (EDL) due sustained hyperemia (intraluminal splitting) chronic overload (abluminal sprouting). Methods: was given i.v. (25 mg ⁄ kg alternate days for 14 days) mice subjected angiogenic stimuli (prazosin synergist extirpation). The responses femoral artery as well capillarity, capillary ultrastructure, levels Rho GTPase were measured. Results: Blood unaffected the sprouting angiotype, but decreased splitting by CA-4-P. In contrast, attenuated capillarity increase both models, associated reduced lamellipodia filopodia formation. Muscle overload, not hyperemia, accompanied an Conclusions: impaired response experimental models. This inhibitory effect a lower splitting, whereas higher activity consistent interruption actin polymerization. Thus, may context-dependent antiangiogenic vivo under conditions.