Broad-Spectrum Biosensor Capable of Detecting and Identifying Diverse Bacterial and Candida Species in Blood
作者： D. Metzgar , M. Frinder , R. Lovari , D. Toleno , C. Massire
DOI: 10.1128/JCM.00966-13
关键词:
摘要: We describe an assay which uses broad-spectrum, conserved-site PCR paired with mass spectrometry analysis of amplicons (PCR/electrospray ionization-mass [ESI-MS]) to detect and identify diverse bacterial Candida species in uncultured specimens. The performance the was characterized using whole-blood samples spiked low titers 64 6 representing breadth coverage assay. had average limit detection 100 CFU bacteria or per milliliter blood, all tested yielded limits between 20 500 milliliter. Over 99% detections correct identifications, whether they were obtained at concentrations well above lowest detectable concentrations. This study demonstrates ability broad-spectrum PCR/ESI-MS assays organisms complex natural matrices that contain high levels background DNA.
sci-hub.se 参考文章(23)
E M Swierkosz, R Flanders, L Melvin, J D Miller, M W Kline, Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. Journal of Clinical Microbiology. ,vol. 27, pp. 1151- 1154 ,(1989) , 10.1128/JCM.27.6.1151-1154.1989
David J Ecker, Rangarajan Sampath, Haijing Li, Christian Massire, Heather E Matthews, Donna Toleno, Thomas A Hall, Lawrence B Blyn, Mark W Eshoo, Raymond Ranken, Steven A Hofstadler, Yi-Wei Tang, New technology for rapid molecular diagnosis of bloodstream infections Expert Review of Molecular Diagnostics. ,vol. 10, pp. 399- 415 ,(2010) , 10.1586/ERM.10.24
Chris D. Crowder, Heather E. Matthews, Steven Schutzer, Megan A. Rounds, Benjamin J. Luft, Oliver Nolte, Scott R. Campbell, Curtis A. Phillipson, Feng Li, Ranga Sampath, David J. Ecker, Mark W. Eshoo, Genotypic Variation and Mixtures of Lyme Borrelia in Ixodes Ticks from North America and Europe PLoS ONE. ,vol. 5, pp. e10650- ,(2010) , 10.1371/JOURNAL.PONE.0010650
M. W. Eshoo, C. D. Crowder, H. Li, H. E. Matthews, S. Meng, S. E. Sefers, R. Sampath, C. W. Stratton, L. B. Blyn, D. J. Ecker, Y.-W. Tang, Detection and Identification of Ehrlichia Species in Blood by Use of PCR and Electrospray Ionization Mass Spectrometry Journal of Clinical Microbiology. ,vol. 48, pp. 472- 478 ,(2010) , 10.1128/JCM.01669-09
J. A. Klappenbach, J. M. Dunbar, T. M. Schmidt, rRNA operon copy number reflects ecological strategies of bacteria. Applied and Environmental Microbiology. ,vol. 66, pp. 1328- 1333 ,(2000) , 10.1128/AEM.66.4.1328-1333.2000
Eva Engvall, The ELISA, Enzyme-Linked Immunosorbent Assay Clinical Chemistry. ,vol. 56, pp. 319- 320 ,(2010) , 10.1373/CLINCHEM.2009.127803
David J. Ecker, Rangarajan Sampath, Christian Massire, Lawrence B. Blyn, Thomas A. Hall, Mark W. Eshoo, Steven A. Hofstadler, Ibis T5000: a universal biosensor approach for microbiology Nature Reviews Microbiology. ,vol. 6, pp. 553- 558 ,(2008) , 10.1038/NRMICRO1918
Steven A. Hofstadler, Rangarajan Sampath, Lawrence B. Blyn, Mark W. Eshoo, Thomas A. Hall, Yun Jiang, Jared J. Drader, James C. Hannis, Kristin A. Sannes-Lowery, Lendell L. Cummins, Brian Libby, Demetrius J. Walcott, Amy Schink, Christian Massire, Raymond Ranken, Jose Gutierrez, Sheri Manalili, Cristina Ivy, Rachael Melton, Harold Levene, Greg Barrett-Wilt, Feng Li, Vanessa Zapp, Neill White, Vivek Samant, John A. McNeil, Duane Knize, David Robbins, Karl Rudnick, Anjali Desai, Emily Moradi, David J. Ecker, TIGER: the universal biosensor International Journal of Mass Spectrometry. ,vol. 242, pp. 23- 41 ,(2005) , 10.1016/J.IJMS.2004.09.014
Jeong Hwan Shin, Raymond Ranken, Susan E. Sefers, Robert Lovari, Criziel D. Quinn, Shufang Meng, Heather E. Carolan, Donna Toleno, Haijing Li, Jeong Nyeo Lee, Charles W. Stratton, Christian Massire, Yi-Wei Tang, Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry Journal of Clinical Microbiology. ,vol. 51, pp. 136- 141 ,(2013) , 10.1128/JCM.01907-12
Carson D Baldwin, Gerald B Howe, Ranga Sampath, Larry B Blyn, Heather Matthews, Vanessa Harpin, Thomas A Hall, Jared J Drader, Steve A Hofstadler, Mark W Eshoo, Karl Rudnick, Karen Studarus, David Moore, Sharon Abbott, J Michael Janda, Chris A Whitehouse, None, Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates. Diagnostic Microbiology and Infectious Disease. ,vol. 63, pp. 403- 408 ,(2009) , 10.1016/J.DIAGMICROBIO.2008.12.012