Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.
作者： Carson D Baldwin , Gerald B Howe , Ranga Sampath , Larry B Blyn , Heather Matthews
DOI: 10.1016/J.DIAGMICROBIO.2008.12.012
关键词:
摘要: Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified the genus level and 115/156 (74%) species level. Only 9% misidentified. This study shows that multilocus PCR/ESI-MS has potential be useful technique identifying broad range bacteria.
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