Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers.

摘要: Abstract Objective Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that expressed in pre-B and T cells. LEF1 also an important member of the Wnt/β-catenin signaling pathway plays roles self-renewal differentiation embryonic stem We speculated might function cells from human exfoliated deciduous teeth (SHED). In this study, we attempted to isolate such LEF1-positive dental pulp (HDDPCs) by genetic engineering technology, using promoter. Design A piggyBac transposon plasmid (pTA-LEN) was introduced into HDDPCs, Neon® transfection system. After G418 selection, emerging colonies were assessed for EGFP-derived fluorescence microscopy. Reverse transcription polymerase chain reaction (RT-PCR) analysis performed RNA isolated these examine cell-specific transcript expression. Osteoblastic or neuronal induced cultivating with differentiation-inducing medium. Results RT-PCR confirmed expression several cell markers, including OCT3/4, SOX2, REX1, NANOG, which could be differentiated osteoblasts Conclusions The HDDPCs exhibited properties cells, suggesting serve as marker SHED.