PATR-A AND B, THE ORTHOLOGUES OF HLA-A AND B, PRESENT HEPATITIS C VIRUS EPITOPES TO CD8+ CYTOTOXIC T CELLS FROM TWO CHRONICALLY INFECTED CHIMPANZEES

摘要: Common chimpanzees (Pan troglodytes) infected with hepatitis C virus (HCV) show a disease progression similar to that observed for human patients. Although most animals develop chronic hepatitis, persistence is associated an ongoing immune response, which the beneficial or detrimental effects are uncertain. Lines of virus-specific cytotoxic CD8+ T lymphocytes (CTL) have been previously established from liver biopsies two common chronically HCV-1. The viral epitopes recognized by six lines CTL defined using synthetic peptides and shown consist 8 9-residue derived various proteins. Five derive sequences vary among strains HCV. majority corresponding variant different HCV were either less efficiently not at all CTL, suggesting their response may limited potential controlling replication variants. Complementary DNAs encoding class I alleles chimpanzees, Patr-A, -B, -C cloned, sequenced, transfected individually into I-deficient cell line. Analysis peptide presentation transfectants identified Patr allotypes present here additional epitope previously. assignment based upon analysis cells correlates precisely segregation antigen-presenting function within panel chimpanzee expression heavy chains as isoelectric focusing. HCV-1 presented Patr-B allotypes, Patr-A allotype, none Patr-C allotypes.