Exploring the utility of cross-laboratory RAD-sequencing datasets for phylogenetic analysis

摘要: Restriction site-Associated DNA sequencing (RAD-Seq) is widely applied to generate genome-wide sequence and genetic marker datasets. RAD-Seq has been extensively utilised, both at the population level across species, for example in construction of phylogenetic trees. However, consistency data generated different laboratories, potential use cross-species orthologous RAD loci estimation relationships, have not investigated. This study describes SbfI evolutionary relationships amongst ten teleost fish using previously established phylogeny as a benchmark. The number identified decreased with increasing distance between several thousand conserved five salmonid species (divergence ~50 MY), hundred more distantly related ~100–360 MY). majority (>70%) were genic origin, suggesting that bias towards regions useful identifying distant orthologs. Interspecific single nucleotide variants each locus identified. Evolutionary estimated concatenated sequences interspecific congruent published phylogenies, even up ~360 MY) species. Overall, this demonstrated can be closely positive implications repeatability its address research questions beyond scope original studies. Furthermore, concordance tree topologies phylogenies suggests similar meta-datasets could utilised prediction populations readily available datasets, but which remain uncharacterised.