In Vitro Neurophysiology

摘要: In vitro studies of neuronal function involve preparing thin slices living brain for experimental study. Brain slice methods have been applied to most regions the brain, however anatomy hippocampus has favored that tissue. are maintained in a life-support chamber from which electrophysiological stimulation and recordings (intra-/extracellular) can be made under visual guidance, often conjunction with application drugs, manipulation ACSF composition, optical recordings, visualization fluorescing markers, subsequent histology. The advantages approaches surgical simplification tissue degree control access methodology provides. Limitations include limited viability, altered phenotype, isolation activating modulating influences. It is anticipated will increasingly used detailed study elements networks identified by functional imaging, effects molecular neurobiological manipulations.