Preparative methods for brain slices: a discussion.

摘要: Criteria for slice health and factors that affect were discussed by many of the participants in conference. In addition to standard parameters (energy metabolism, morphology, electrophysiological responsiveness) more subtle but possibly equally important manifestations discussed. These included protein synthesis, changes, which we are becoming increasingly aware. The latter include synthesis stress-related proteins, altered levels phosphorylation, proteolysis. last only touched on, it is apparent they do fact constitute differences between preparation vivo tissue. They may well lead quite different responses slices from those occur vivo. While ways optimizing wellness discussed, there was a fair consensus certain adjustments will optimize most widely measured aspects cell function. following, wherever possible. Use young animals, use interface chamber, preparing with vibratome, pre-treating animals ice-cold cardiac perfusion before sacrificing, using pre-incubation media reduce NMDA receptor activation, free radical formation swelling. When possible these treatments should perhaps be continued into normal incubation. This being said, viewpoints actually expressed Discussion, read get feel usefulness approaches.