In vitro growth and differentiation of primary follicles isolated from cryopreserved sheep ovarian tissue

摘要: Abstract Achieving full in vitro growth of oocytes both domestic animals and humans remains a major challenge. The objective this study was to examine the development primary follicles isolated enzymatically from cryopreserved sheep ovarian tissue. In Experiment 1, (mean diameter 60.1 ± 0.78 μm) were cultured serum-free medium on fibronectin-coated wells for 42 days. Initially follicular structure lost as granulosa cells plated down, but by Day 7 two distinct morphologies began emerge. Nineteen out 36 gradually re-surrounded cells, forming follicle-like units (reorganized follicles), remaining 17 not (non-reorganized follicles). On 2, there no difference between reorganized non-reorganized follicles. (mean ± S.E.M.) increased ( P days) examined. Oocytes reached maximum 75.4 ± 2.0 μm, size equivalent that ovine secondary Using RT-PCR, mRNA follicle stimulating hormone receptor detected freshly long-term follicles, 3, we tested if culture conditions could support further oocyte 77.7 ± 1.6 μm 98.8 ± 2.1 μm