Isolation of Bacillus licheniformis Producing Antimicrobial Agents against Bacillus cereus and Its Properties

摘要: In order to manufacture Bacillus cereus-free fermented soybean products, an antimicrobial agentproducing isolate against B. cereus was obtained from 150 traditionally products. The morphological and biochemical tests the phylogenetic relationship among 16S rRNA gene sequences indicated that named as strain SCK 121057 most closely related licheniformis. licheniformis began produce agent after 48 h of incubation. nonproteinaceous insensitive heat, long term storage protease K. Electron microscopic observation attacked membrane cereus, leaving ghost cell. inhibited growth subtilis, Lactobacillus brevis various types pathogenic strains including Escherichia coli, E. faecalis, Micrococcus luteus, Staphylococcus aureus, Aspergillus flavus, A. ochraceus, parasiticus well cereus. After coinoculation in ratio (as basis CFU/g sample) 10 1 on surface cooked soybeans, cell numbers had been dramatically reduced 31 days incubation compared those single inoculation