The PlcR Virulence Regulon of Bacillus cereus

摘要: PlcR is a Bacillus cereus transcriptional regulator, which activates gene expression by binding to nucleotidic sequence called the ‘PlcR box’. To build list of all genes included in regulon, consensus was identified directed mutagenesis. The reference strain ATCC14579 sequenced genome searched for occurrences this produce virtual regulon. control these confirmed comparing and its isogenic Δ-plcR using DNA microarrays, lacZ fusions proteomics methods. resulting 45 controlled 28 boxes. Forty proteins were exported, 22 secreted extracellular medium 18 bound or attached cell wall structures (membrane peptidoglycan layer). functions related food supply (phospholipases, proteases, toxins), protection (bacteriocins, toxins, transporters, biogenesis) environment-sensing (two-component sensors, chemotaxis proteins, GGDEF family regulators). Four coded cytoplasmic regulators. regulon appears integrate large range environmental signals, including deprivation self cell-density, regulate transcription designed overcome obstacles that hinder B. growth within host: supply, host barriers, immune defenses, competition with other bacterial species. be key component efficient adaptation environment.