Method for detecting colon cancer markers

作者: Shigeru Kanaoka

DOI:

关键词:

摘要: A method of detecting a tumor marker for the diagnosis large bowel cancer and adenomatous polyposis coli comprising steps a) collecting freezing feces, b) homogenizing frozen feces in presence an RNAase inhibitor preparing suspension, c) extracting RNA from obtained sample which is extracted, d) obtaining cDNA by reverse transcribing extracted RNA, e) amplifying cDNA, f) amplified characterized that one or more markers selected group consisting COX-2, SNAIL MMP-7 (with proviso case where only COX-2 excluded).

参考文章(6)
Robert S. Chapkin, Joanne R. Lupton, Laurie A. Davidson, Noninvasive detection of colonic biomarkers using fecal messenger RNA ,(1998)
Piotr Chomczynski, Product and process for isolating RNA ,(1987)
George Cimino, Yu S. Zhu, Lily Lin, Nucleic acid preparation methods ,(1994)
Martin J. Heslin, Jieming Yan, Martin R. Johnson, Heidi Weiss, Robert B. Diasio, Marshall M. Urist, Role of matrix metalloproteinases in colorectal carcinogenesis. Annals of Surgery. ,vol. 233, pp. 786- 792 ,(2001) , 10.1097/00000658-200106000-00008
Anthony P. Shuber, Stanley N. Lapidus, Gail E. Radcliffe, Methods for stool sample preparation ,(1998)