作者: Jeanette N. McClintick , Jay A. Tischfield , Li Deng , Manav Kapoor , Xiaoling Xuei
DOI: 10.1016/J.ALCOHOL.2019.01.001
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摘要: Abstract The short-term effects of alcohol on gene expression in brain tissue cannot directly be studied humans. Because neuroimmune signaling is altered by alcohol, immune cells are a logical, accessible choice to study and may provide biomarkers. RNAseq was used the 48-h exposure ethanol lymphoblastoid cell lines (LCLs) from 20 alcoholic subjects control subjects. Ethanol resulted differential 4456 12,503 genes detectably expressed LCLs (FDR [false discovery rate] ≤ 0.05); 52% these showed increased expression. Cells responded similarly. whose changed fell into many pathways: NFκB, neuroinflammation, IL6, IL2, IL8, dendritic maturation pathways were activated, consistent with TNF, IL1, IL4, IL18, TLR4, LPS. Signaling Interferons A B decreased, as did EIF2 signaling, phospholipase C glycolysis. Baseline patterns similar At relaxed stringency (p show exposure, some which might biomarkers for use disorders. Identifying can aid interpreting within loci identified GWAS play functional roles.