Gene-activated fat grafts for the repair of spinal cord injury: a pilot study.
作者： Volker M. Betz , K. Hakan Sitoci-Ficici , Ortrud Uckermann , Elke Leipnitz , Anne Iltzsche
DOI: 10.1007/S00701-015-2626-Y
关键词:
摘要: Spinal cord injury (SCI) is a complex disease requiring concerted multi-target approach. The most appropriate combination of therapeutic gene, cellular vehicle, and space filling scaffold still has to be determined. We present an approach that employs syngeneic adipose tissue serving as three-dimensional biological implant, source progenitor cells, delivery system for genes. In this pilot experiment, we evaluated the feasibility short-term effects using gene-activated autologous fat grafts after SCI. An experimental SCI model was established in Fischer 344 rats by T9-T10 hemimyelonectomy. Fat harvested from two donor rats. Animals were divided into four groups treated with either (i) activated adenoviral vector carrying human NT-3 cDNA, (ii) or BDNF, (iii) untreated (iv) remained untreated. euthanized 7 21 days surgery, spinal investigated histological immunohistochemical methods. BDNF produced at least vitro vivo. stable site implantation 7 days 21 days. Neither BDNF-activated nor NT-3-activated graft had detectable limiting effect on neuronal degeneration. recruited microglia perilesional attenuated their inflammatory response. Gene-activated serves material delivering molecules over extended period time. BDNF-fat Whether these findings translate functional recovery will require observation times.
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