Expression of a β-Lactamase Preproinsulin Fusion Protein in Escherichia Coli
作者： Anne W. Emerick , Barbara L. Bertolani , A. Ben-Bassat , Thomas J. White , Michael W. Konrad
DOI: 10.1038/NBT0284-165
关键词:
摘要: The level of expression a β-lactamase human preproinsulin fusion protein1 in Escherichia coli was increased by plasmid and host manipulations. initial (0.01% total protein) two- to three-fold replacing the p-lactamase promoter with two strong promoters: trp from Serratia marcescens lacUV5 E. coli. Replacement ribosome binding site (RBS) RBS enhanced yield 0.16% cell protein. Proinsulin unstable, half-life 14 minutes at 37°C. (to about 0.5% incubating 30°C rather than
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