Functional human antigen-specific T cells produced in vitro using retroviral T cell receptor transfer into hematopoietic progenitors.
作者： Anja U. van Lent , Maho Nagasawa , Marleen M. van Loenen , Remko Schotte , Ton N. M. Schumacher
DOI: 10.4049/JIMMUNOL.179.8.4959
关键词:
摘要: In vitro production of human T cells with known Ag specificity is major clinical interest for immunotherapy against tumors and infections. We have performed TCRalphabeta gene transfer into hemopoietic progenitors from postnatal thymus or umbilical cord blood, subsequently cultured these precursors on OP9 stromal expressing the Notch ligand Delta-like1. report here that fully mature, functional controlled are obtained such cultures. Using vectors encoding TCRalphabeta-chains directed melanoma (MART-1), viral (CMV), minor histocompatibility (HA-2) Ags, we show Ag-specific exert cytolytic activity their cognate expand in upon specific TCR stimulation. Therapeutic applications may arise results because they provide a way to produce large numbers autologous mature undifferentiated progenitors.
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