Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice.

摘要: Although T-cell receptor (TCR) transgenic as well knockout and knockin mice have had a large impact on our understanding of development, signal transduction function, the need to cross these delays experiments considerably. Here we provide methodology for rapid expression TCRs in using 2A peptide-linked multicistronic retroviral vectors transduce stem cells any background before adoptive transfer into RAG-1(-/-) mice. For simplicity, refer retrogenic We demonstrate that are comparable expressing three commonly used (OT-I, OT-II [corrected] AND). also show male antigen-specific (HY, MataHari Marilyn) facilitated analysis positive negative selection female mice, respectively. examined various tolerance mechanisms epitope-coupled TCR This powerful resource could expedite identification proteins involved development function.