Secreted Frizzled-Related Protein 5 Attenuates High Phosphate-Induced Calcification in Vascular Smooth Muscle Cells by Inhibiting the Wnt/ß-Catenin Pathway
作者： Dai Deng , Zongli Diao , Xue Han , Wenhu Liu
DOI: 10.1007/S00223-016-0117-7
关键词:
摘要: Vascular calcification (VC) is highly prevalent and represents a major cardiovascular risk factor in chronic kidney disease (CKD) patients. High phosphate (HP) levels are strongly associated with VC this population. Secreted frizzled-related protein 5 (SFRP5), one of the inhibitors Wnt pathway, known anti-inflammatory adipokine positive effect on metabolic diseases, addition to its anticancer potency. However, role SFRP5 pathophysiology unclear. This work aimed study mechanism action progression HP-induced VC, which resembles CKD-related through direct vascular smooth muscle cells (VSMCs) vitro. Addition significantly inhibited VSMCs as determined by Alizarin red staining calcium content. The inhibitory was due suppression expression osteoblastic markers. In addition, abrogated activation Wnt/s-catenin plays key pathogenesis VC. specificity for inhibition confirmed using neutralizing antibody SFRP5. Our results suggest that inhibits inhibiting markers, well pathway. may indicate potential therapeutic agent VSMCs.
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