Juvenile hormone‐regulated locust vitellogenin genes: Lack of expression after transfer into Drosophila

摘要: In Locusta migratoria, vitellogenin (Vg) is normally produced only in adult female fat body under stimulation by juvenile hormone (JH). This permits study of (a) programming genes for expression and (b) modulation JH. From L. migratoria genomic libraries, two Vg (A B) have been cloned. Coding regions encompass 10-12 kbases DNA, contain introns, hybridize with 6,300 nucleotide mRNAs. Although the 5'-exons, coding signal peptides, are similar sequence, remaining sequences distinct restriction maps show no crosshybridization. Upstream DNA from has some sequence similarity, corresponding to potential regulatory regions. Dot hybridization assays mRNAs A B locust after induction methoprene coordinate also a third, unidentified JH-regulated gene. hope providing system identification control sequences, P element-mediated transformation used transfer into Drosophila. gene B, central block was deleted, give mini-Vg comprising 5' 3' terminal plus upstream flanking DNA. incorporated element vector Carnegie 20 injected Drosophila embryos. Three transformed fly lines were obtained which integrated at different chromosomal sites. On Northern blots RNA, however, could be detected, even though accompanying rosy expressed. suggests that not recognized Drosophila, current effort directed toward developing homologous assay cloned genes.