The non-histone proteins of chromatin

摘要: Abstract 1. A method is described for the fractionation of salt-urea-dissociated chromatin using hydroxylapatite. With exception experiments chromatins prepared from “citric acid” nuclei, high yields acidic non-histone proteins, relatively free RNA, can be obtained by this procedure. 2. The proteins a number were compared electrophoresis in sodium dodecyl sulphate-urea polyacrylamide gels employing discontinuous buffer system. Proteins mouse nuclei found to extremely heterogeneous, but case calf thymus mainly low molecular weight. On other hand, “sucrose” appeared contain fewer weight species tissues studied, with brain. Preparation double-detergent procedure Penman (J. Mol. Biol., 17 (1966) 117) gave protein DNA ratio. These also predominantly Duck erythrocyte lysis contained proteins. 3. Using salt techniques attempts made remove “cytoplasmic” and “residual” chromatin. which remained histones kidney liver, brain wide spectrum was seen. 4. Little tissue or specificity on comparison bovine tissues. 5. It concluded that remain tightly bound are same approximate size as basic histones. Because procedural variation heterogeneity these detection single regulatory appears require techniques.