Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer?

摘要: Recent studies indicate that circulating microRNAs in serum/plasma are a novel class of non-invasive biomarkers with diagnostic and prognostic information. So far, have not been analyzed patients bladder cancer. We collected serum from non-muscle invasive cancer (NMIBC), muscle (MIBC) non-malignant urological disease. Total RNA was isolated 400 μl using the mirVana PARIS Kit; artificial cel-miR-39 spiked-in prior to isolation control different efficiencies. Quantitative real-time PCR applied measure levels 22 upregulated BCA tissue (miR-15a, miR-18a, miR-21, miR-93, miR-96, miR-103, miR-130b, miR-135b, miR-141, miR-182, miR-183, miR-190, miR-191, miR-200b, miR-422b, miR-425, miR-449b, miR-601, miR-639, miR-644, miR-649 miR-1233) marker identification cohort (NMIBC, n = 11, MIBC, n = 10; controls, n = 10). The most promising were tested validation n = 65, n = 61; n = 105). recovery similar NMIBC, MIBC subjects. analysis microRNA indicated miR-141 miR-639 increased compared CTRL. these demonstrated Furthermore, correlated clinicopathological parameters (pT-stage, metastasis, grading). does seem be helpful diagnosis or prognosis BCA.