Isolation of oligogalacturonic acids up to DP 20 by preparative high-performance anion-exchange chromatography and pulsed amperometric detection.
作者： Arland T. Hotchkiss , Stephanie L. Lecrinier , Kevin B. Hicks
DOI: 10.1016/S0008-6215(01)00170-7
关键词:
摘要: Milligram quantities of oligogalacturonic acids up to a degree polymerization (DP) 20 were purified by high-performance anion-exchange chromatography utilizing preparative-scale (21-mm i.d.) CarboPac PA1 column and nonlinear potassium acetate (pH 7.5) gradient. Detection was accomplished pulsed amperometry without post-column addition hydroxide. Pulsed at near-neutral pH is an excellent detection method for preparative separations carbohydrates because it avoids base-catalyzed degradation reactions that can occur high pH. This simpler, faster, had higher sample loading capacity allowed the isolation DP than other methods reported previously. With this improved method, multi-milligram valuable (up 20) be readily isolated.
elsevier.com
sci-hub.se 参考文章(19)
Arland T. Hotchkiss, Karimah El-Bahtimy, Marshall L. Fishman, Analysis of Pectin Structure by HPAEC-PAD Springer, Berlin, Heidelberg. pp. 129- 146 ,(1996) , 10.1007/978-3-642-60989-3_8
E. Melotto, L. C. Greve, J. M. Labavitch, Cell Wall Metabolism in Ripening Fruit (VII. Biologically Active Pectin Oligomers in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits). Plant Physiology. ,vol. 106, pp. 575- 581 ,(1994) , 10.1104/PP.106.2.575
Kevin B. Hicks, Scott M. Sondey, Preparative high-performance liquid chromatography of malto-oligosaccharides Journal of Chromatography A. ,vol. 389, pp. 189- 194 ,(1987) , 10.1016/S0021-9673(01)94422-X
Henk A. Schols, Alfons G.J. Voragen, Ian J. Colquhoun, Isolation and characterization of rhamnogalacturonan oligomers, liberated during degradation of pectic hairy regions by rhamnogalacturonase Carbohydrate Research. ,vol. 256, pp. 97- 111 ,(1994) , 10.1016/0008-6215(94)84230-2
Mark D. Spiro, Keith A. Kates, Alan L. Koller, Malcolm A. O'Neill, Peter Albersheim, Alan G. Darvill, Purification and characterization of biologically active 1,4-linked α-d-oligogalacturonides after partial digestion of polygalacturonic acid with endopolygalacturonase Carbohydrate Research. ,vol. 247, pp. 9- 20 ,(1993) , 10.1016/0008-6215(93)84237-Z
M. R. Hardy, R. R. Townsend, Separation of positional isomers of oligosaccharides and glycopeptides by high-performance anion-exchange chromatography with pulsed amperometric detection. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 3289- 3293 ,(1988) , 10.1073/PNAS.85.10.3289
Arland T. Hotchkiss, Kevin B. Hicks, Landis W. Doner, Peter L. Irwin, Isolation of oligogalacturonic acids in gram quantities by preparative h.p.l.c. Carbohydrate Research. ,vol. 215, pp. 81- 90 ,(1991) , 10.1016/0008-6215(91)84009-4
S. C. Fry, S. Aldington, P. R. Hetherington, J. Aitken, Oligosaccharides as signals and substrates in the plant cell wall. Plant Physiology. ,vol. 103, pp. 1- 5 ,(1993) , 10.1104/PP.103.1.1
Jinhua An, Li Zhang, Malcolm A. O'Neill, Peter Albersheim, Alan G. Darvill, Isolation and structural characterization of endo-rhamnogalacturonase-generated fragments of the backbone of rhamnogalacturonan I Carbohydrate Research. ,vol. 264, pp. 83- 96 ,(1994) , 10.1016/0008-6215(94)00186-3
Piet J.H. Daas, Karin Meyer-Hansen, Henk A. Schols, Gerhard A. De Ruiter, Alphons G.J. Voragen, Investigation of the non-esterified galacturonic acid distribution in pectin with endopolygalacturonase Carbohydrate Research. ,vol. 318, pp. 135- 145 ,(1999) , 10.1016/S0008-6215(99)00093-2