Analysis of methylated and unmethylated polygalacturonic acid structure by polysaccharide analysis using carbohydrate gel electrophoresis.
作者： Florence Goubet , Brooke Morriswood , Paul Dupree
DOI: 10.1016/S0003-2697(03)00438-X
关键词: Polyacrylamide 、 Polysaccharide 、 Pectin 、 Electrophoresis 、 Cell wall 、 Biochemistry 、 Methylation 、 Chromatography 、 Chemistry 、 Hydrolysis 、 Gel electrophoresis
摘要: The electrophoretic migration in polyacrylamide gels of oligogalacturonic acids (OGAs) derivatized by a fluorophore (2-aminoacridone) was studied. We found conditions such that OGAs can be separated up to degree polymerization (DP) 40. dependent on methylation and DP, because the OGA mobility relies charge galacturonic acid residues. Since both methylated unmethylated oligosaccharides resolved, polysaccharide analysis using carbohydrate gel electrophoresis (PACE) is powerful method for studying fingerprint pectin hydrolysis. It used characterize endopolygalacturonase (Endo-PG) tolerance methylation. Furthermore, an Endo-PG distinguish low highly pectin, PACE investigate blockwise or nonblockwise distribution polygalacturonic acid. show applied crude cell wall preparations Arabidopsis inflorescence stems. Using chemical deesterification before after digestion, we wall, pectins have nonesterified esterified regions.
elsevier.com
sci-hub.se 参考文章(27)
Stephen C. Fry, The Growing Plant Cell Wall: Chemical and Metabolic Analysis ,(1988)
Jan. Sudor, Milos V. Novotny, End-label, free-solution capillary electrophoresis of highly charged oligosaccharides. Analytical Chemistry. ,vol. 67, pp. 4205- 4209 ,(1995) , 10.1021/AC00118A026
Pauline M Rudd, Raymond A Dwek, Rapid, sensitive sequencing of oligosaccharides from glycoproteins. Current Opinion in Biotechnology. ,vol. 8, pp. 488- 497 ,(1997) , 10.1016/S0958-1669(97)80073-0
Tadashi Ishii, Junji Ichita, Hajime Matsue, Hiroshi Ono, Ikuko Maeda, Fluorescent labeling of pectic oligosaccharides with 2-aminobenzamide and enzyme assay for pectin Carbohydrate Research. ,vol. 337, pp. 1023- 1032 ,(2002) , 10.1016/S0008-6215(02)00087-3
Arland T. Hotchkiss, Stephanie L. Lecrinier, Kevin B. Hicks, Isolation of oligogalacturonic acids up to DP 20 by preparative high-performance anion-exchange chromatography and pulsed amperometric detection. Carbohydrate Research. ,vol. 334, pp. 135- 140 ,(2001) , 10.1016/S0008-6215(01)00170-7
J.Paul Knox, PaulJ. Linstead, Janet King, Christine Cooper, Keith Roberts, Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. ,vol. 181, pp. 512- 521 ,(1990) , 10.1007/BF00193004
Ramchandra V Gadre, Gonzalez Van Driessche, Jozef Van Beeumen, Mahalingeshwara K Bhat, Purification, characterisation and mode of action of an endo-polygalacturonase from the psychrophilic fungus Mucor flavus Enzyme and Microbial Technology. ,vol. 32, pp. 321- 330 ,(2003) , 10.1016/S0141-0229(02)00291-0
Piet J.H. Daas, Karin Meyer-Hansen, Henk A. Schols, Gerhard A. De Ruiter, Alphons G.J. Voragen, Investigation of the non-esterified galacturonic acid distribution in pectin with endopolygalacturonase Carbohydrate Research. ,vol. 318, pp. 135- 145 ,(1999) , 10.1016/S0008-6215(99)00093-2
Jun Fu, Rolf Prade, Andrew Mort, Expression and action pattern of Botryotinia fuckeliana (Botrytis cinerea) rhamnogalacturonan hydrolase in Pichia pastoris. Carbohydrate Research. ,vol. 330, pp. 73- 81 ,(2001) , 10.1016/S0008-6215(00)00268-8
Florence Goubet, Peter Jackson, Michael J. Deery, Paul Dupree, Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases. Analytical Biochemistry. ,vol. 300, pp. 53- 68 ,(2002) , 10.1006/ABIO.2001.5444