Rapid, sensitive sequencing of oligosaccharides from glycoproteins.
作者： Pauline M Rudd , Raymond A Dwek
DOI: 10.1016/S0958-1669(97)80073-0
关键词: Glycoprotein 、 Polyacrylamide gel electrophoresis 、 High-performance liquid chromatography 、 Mass spectrometry 、 Chromatography 、 Exoglycosidase 、 Chemistry
摘要: The release of sub-picomole levels N-linked oligosaccharides directly from 1–5 μg protein in a band on an SDS PAGE gel, coupled with recent advances mass spectrometry and HPLC, opens the way for analysis biologically important glycoproteins that are difficult to purify or available only limited amounts. A straightforward HPLC strategy enables structures both neutral sialylated components N-glycan pool be predicted single run. entire sugars may then sequenced simultaneously, using exoglycosidase arrays.
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