How Nucleic Acids Cope with High Temperature

摘要: This chapter discusses the question of coping up nucleic acids with high temperature at polynucleotide level—RNA, DNA, and their ribonucleoprotein derivatives (RNP/DNP). When are heated in aqueous solution, two types phenomena take place: denaturation architecture chemical degradation building blocks. In vivo, half-lives both RNA DNA thermophilic organisms usually longer than that estimated vitro, attesting to cellular strategies protecting against deleterious effects heat. Despite susceptibility certain modified bases ribonucleotide chain thermal degradation, most naturally occurring tRNAs (especially those from hyperthermophilic organisms) appear fairly resistant heat denaturation. intrinsic potentiality degrade elevated temperatures, many hyperthermophiles can survive very temperatures approaching or even surpassing boiling point water. The majority stable RNAs, such as tRNA rRNA molecules, contain a variety nucleosides. Stabilizing RNAs DNAs may be classified into three major categories: (i) which structures acids; (ii) dependent on extrinsic interactions other biomolecules; (iii) battery enzymes for detecting repairing damage constantly renew functional molecules. Genetic approach using mutant strains mutated one more biomolecules supposedly involved directly indirectly stabilization should systematically used.