Unique Distribution of Diacyl-, Alkylacyl-, and Alkenylacyl-Phosphatidylcholine Species Visualized in Pork Chop Tissues by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging.

摘要: Phosphatidylcholine (PC) is the major phospholipid in meat and influences qualities, such as healthiness. PC classified into three groups based on bond at sn-1 position: Diacyl, alkylacyl, alkenylacyl. To investigate their composition distribution pork tissues, including longissimus thoracis et lumborum (loin) spinalis muscles, intermuscular fat, transparent we performed matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI–MSI). Eleven diacyl-, seven alkylacyl-, six alkenylacyl-PCs were identified using liquid chromatography (LC)-tandem MS (MS/MS) analysis. Despite many alkylacyl- alkenylacyl-PC species sharing identical m/z values, able to visualize these MALDI–MSI. Diacyl- and/or showed unique patterns suggesting that dependent fatty acid compositions. PCs are a dietary source of choline meat, amount was significantly higher muscle tissues. Consumption mitigates age-related memory decline neurodegenerative diseases; therefore, consumption tissues could help mitigate diseases. These results support use MALDI–MSI analysis for assessing association between quality parameters meat.