The mitochondrial uncoupling protein gene. Correlation of exon structure to transmembrane domains.
作者： L P Kozak , J H Britton , U C Kozak , J M Wells
DOI: 10.1016/S0021-9258(18)37751-2
关键词:
摘要: The mitochondrial uncoupling protein, a protein essential for the thermogenic properties of brown fat in mammals, is inserted inner membrane by means six alpha-helical hydrophobic transmembrane domains. We have sequenced complete cDNA and parts gene to determine that composed exons, each which encodes domain. also show transcription from single start site; however, use alternative poly(A) addition signal sequences results two mRNAs, major species 1221 nucleotides, not including tail, minor about 1600 nucleotides. 5′-untranslated region mRNA 231 3′-untranslated contains 81 nucleotides prior tail.
sci-hub.st 参考文章(21)
H. Aquila, T.A. Link, M. Klingenberg, The uncoupling protein from brown fat mitochondria is related to the mitochondrial ADP/ATP carrier. Analysis of sequence homologies and of folding of the protein in the membrane. The EMBO Journal. ,vol. 4, pp. 2369- 2376 ,(1985) , 10.1002/J.1460-2075.1985.TB03941.X
M. J. Runswick, S. J. Powell, P. Nyren, J. E. Walker, Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondria uncoupling protein. The EMBO Journal. ,vol. 6, pp. 1367- 1373 ,(1987) , 10.1002/J.1460-2075.1987.TB02377.X
M A Glotzer, U Stadler, L P Kozak, A Jacobsson, Mitochondrial uncoupling protein from mouse brown fat: molecular cloning, genetic mapping, and mRNA expression Journal of Biological Chemistry. ,vol. 260, pp. 16250- 16254 ,(1985) , 10.1016/S0021-9258(17)36228-2
L Liscum, J Finer-Moore, R M Stroud, K L Luskey, M S Brown, J L Goldstein, Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum. Journal of Biological Chemistry. ,vol. 260, pp. 522- 530 ,(1985) , 10.1016/S0021-9258(18)89764-2
F Bouillaud, J Weissenbach, D Ricquier, Complete cDNA-derived amino acid sequence of rat brown fat uncoupling protein. Journal of Biological Chemistry. ,vol. 261, pp. 1487- 1490 ,(1986) , 10.1016/S0021-9258(17)35962-8
B. K. Kobilka, R. A. Dixon, T. Frielle, H. G. Dohlman, M. A. Bolanowski, I. S. Sigal, T. L. Yang-Feng, U. Francke, M. G. Caron, R. J. Lefkowitz, cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 46- 50 ,(1987) , 10.1073/PNAS.84.1.46
Robert G. Ridley, Hasmukh V. Patel, Gerhard E. Gerber, Robert C. Morton, Karl B. Freeman, Complete nucleotide and derived amino acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of a mitochondrial targeting presequence Nucleic Acids Research. ,vol. 14, pp. 4025- 4035 ,(1986) , 10.1093/NAR/14.10.4025
J Corden, B Wasylyk, A Buchwalder, P Sassone-Corsi, C Kedinger, P Chambon, Promoter sequences of eukaryotic protein-coding genes Science. ,vol. 209, pp. 1406- 1414 ,(1980) , 10.1126/SCIENCE.6251548
J. Geliebter, R. A. Zeff, R. W. Melvold, S. G. Nathenson, Mitotic recombination in germ cells generated two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbm6. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 3371- 3375 ,(1986) , 10.1073/PNAS.83.10.3371
W. Benton, R. Davis, Screening lambdagt recombinant clones by hybridization to single plaques in situ Science. ,vol. 196, pp. 180- 182 ,(1977) , 10.1126/SCIENCE.322279