Do cancer cells have distinct adhesions in 3D collagen matrices and in vivo

摘要: Abstract During metastasis, cancer cells breach the basement membrane and migrate through stroma mostly composed of a network collagen I fibers. Cell migration on 2D is initiated by protrusion cell followed formation adhesions that link actin cytoskeleton to extracellular matrix (ECM). Cells then move forwards exerting traction forces at its front disassembling rear. In 2D, only ventral surface migrating in contact with ECM, where cell–matrix are assembled. 3D matrices, even though whole available for interacting it unclear whether discrete adhesion structures actually exist. Using high-resolution confocal microscopy we imaged endogenous adhesome proteins three different types embedded non-pepsinized type I, polymerized slow rate, allow resembles organization EMC observed vivo. Vinculin aggregates were detected cellular protrusions, frequently colocalizing fibers, implying they correspond 3D. As distance from substrate bottom increases, become smaller almost undetectable some lines. intravital imaging show here, first time, existence These share similarities ones found matrices. It still remains be determined if assembled vivo functional well-described 2D. This will provide major step forward understanding more physiological environments.