Basement membrane invasion assays: native basement membrane and chemoinvasion assay.

摘要: To escape the primary tumor and infiltrate stromal compartments, invasive cancer cells must traverse basement membrane (BM). break this dense matrix, develop finger-like protrusions, called invadopodia, at their ventral surface. Invadopodia secrete proteases to degrade BM, then elongate which allows cell invade subjacent tissue. Here, we describe two complementary invasion assays. The native BM assay, based on isolated from rat or mouse mesentery, is a physiologically significant approach for studying stages of crossing cellular level. Matrigel-based chemoinvasion assay powerful technique invadopodia's molecular composition organization subcellular