Quantitative measurement of hypermodified adenosine ribonucleosides in plant tissues

作者: Chong-Maw Chen , Gary F. Hartnell , O'Brien C. Smith , Walter S. Francis

DOI: 10.1016/0003-2697(74)90253-X

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摘要: Abstract A procedure for analyzing subnanomole levels of hypermodified ribonucleosides in small amounts plant tissue is described. It involves sodium metaperiodate oxidation, tritiated borohydride reduction, and separation nucleosides by Sephadex LH-20 column 35% ethanol followed paper chromatography. Data the identification modified glc chromatography are included.

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