Isolation of 80S ribosomes from spores of Aspergillus fumigatus and their antigenicity in rabbits
作者： M. Phillips , J. R. Thurston , K. A. Brogden , J. L. Richard
DOI: 10.1007/BF00518802
关键词:
摘要: Ribosomes were obtained from spores of Aspergillus fumigatus by mechanical disruption and differential centrifugation. The initial preparation (crude ribosomes) contained spore components which appeared to be broken fragments plasmalemma with or without organelles. Purified ribosomes free membranous material prepared gel filtration chromatography on Sepharose CL-4B. Monomeric 80S consisting 40% protein 60% RNA, morphologically characteristic fungal isolated.
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