Placental protein 13 (PP-13): effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies.

摘要: Abstract Placental tissue protein 13 (PP-13), one of the 56 known placental proteins identified till today, was purified from placentas obtained women at delivery, and used to evoke antibodies against it. The PP-13 lysed peptides, which were sequenced, leading full-length cDNA sequencing its expression in Escherichia coli . Sequence analysis databases showed homology galectin family. Of various antibody preparations developed, a pair monoclonal (MAbs) coupled recombinant (PP-13-R) for immunodetection pregnant women's serum with solid-phase ELISA format. With dynamic range 25–500pg/mL no background non-pregnant men's serum, test suitable detection 1st, 2nd, 3rd trimesters. levels slowly increase during pregnancy. In 1st trimester, lower than normal found fetal growth restriction (IUGR), preeclampsia (PE), particularly early PE ( Application cultured trophoblasts elicited depolarization carried by calcium ions, followed liberation linoleic arachidonic acids trophoblast membrane, subsequent elevation prostacyclin thromboxane. These effects negligible when derived patients IUGR, or PTD used. results are discussed view potential utilization screening assess risk develop insufficiency, differential pathologies analyzing trophoblasts.