Significance of incorporating measures of sperm production and function into rat toxicology studies.

摘要: The rat is the preferred species for reproductive toxicity testing. inclusion of measures sperm quality, such as motility and morphology, into test protocols often increases sensitivity to detect effects, provides toxicologist risk assessor with valuable information about nature substance. Technical advances in computer-aided analysis have made it possible evaluate motion characteristics spermatozoa. This technology can provide an objective means classifying spermatozoa progressive or non-progressive, required protocols. More specific tests function are being applied purpose evaluating modes mechanisms toxicant action. Computer-aided be used during cultures that support capacitation identify hyperactivated Under same culture conditions, acrosome-specific stains effects toxicants on acrosome reaction. These approaches, combination vitro fertilization rats, pinpoint functional deficits thereby assist addressing hypotheses regarding cellular-molecular bases toxicant-induced male infertility.