α-Tocopherol-anchored gemini lipids with delocalizable cationic head groups: the effect of spacer length on DNA compaction and transfection properties.
作者： Srilakshmi V Patri , Mallikarjun Gosangi , Hithavani Rapaka , Venkatesh Ravula
DOI: 10.1039/D1OB00475A
关键词:
摘要: Understanding the role of structural units in cationic lipids used for gene delivery is essential designing efficient vehicles. Herein, we report a systematic structure–activity investigation on influence spacer length DNA compaction ability and transfection properties gemini with delocalizable head groups. We have synthesized series dimeric varying length. The binding interactions liposomal formulations were characterized by gel electrophoresis ethidium bromide (EtBr) exclusion assays. Condensation potentials optimized best results observed possessing 6 methylene (TIM 6). found that size lipid/DNA complex decreased increase chain up to TIM increased further. lipid/DOPE molar formulation using β-galactosidase activity assay ratio 1 : 1.5 (gemini lipid/DOPE) showed maximum among all ratios. cellular uptake co-localization lipoplexes cell analysis imaging confocal microscopy. confirm lipoplex derived from lipid pCMV-bgal/DNA internalizes via endocytosis. cytotoxicity studies MTT revealed show comparable viability commercial standard even at higher charge Overall, data suggest these dimers depends containing six aliphatic has potential deliver genes.
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