New procedures and parameters for better evaluation of Androctonus Australis garzonii (Aag) and Buthus occitanus tunetanus (Bot) scorpion envenomations and specific serotherapy treatment

摘要: Abstract New procedures describing intoxication with variable amounts of scorpion venoms (from 1 to 5 ld 50 ) allowed us introduce new parameters evaluate Aag and Bot envenomations. Significant differences between the fatal limit time (FLT) last mortality (LMT) were observed when amount venom injected was equal or higher than 2 . For , percentage fast (FM) delayed (DM) varied conversely increased from The relationship 20), median protective dose ( pd neutralizing activity specific antivenom have been established. in a parallel manner titres /ml) decreased 74 ± 3 44 that 52 36 number 20. Antivenom potency evaluated using different protocols based on presence absence preincubation antivenom. In experiments where simultaneously but immediately injected, twice as high those found preincubated (30 min at 37 °C). On contrary, corresponding two times lower. an attempt simulate accidental envenomations subsequent serotherapy, (4 subcutaneously appropriate s intravenously administered intervals after envenomation. When administration shorter FLT, all envenomed mice might be protected by increasing volume However, is closer FLT only 60% envenomed, respectively, could saved even more injected.