The immune responses and antioxidant status of Portunus trituberculatus individuals with different body weights.
作者： Xianyun Ren , Xuan Yu , Baoquan Gao , Jian Li , Ping Liu
DOI: 10.1016/J.FSI.2016.03.004
关键词:
摘要: Vibrio alginolyticus is a virulent pathogen that affects crab aquacultures. In the present study, immune responses and antioxidant status of big small (based on body weight size) 80-, 100- 120-day-old specimens Portunus trituberculatus, challenged for 72 h with alginolyticus, were studied. The total hemocyte count (THC), phagocytic, prophenoloxidase phenoloxidase activities, individuals (BIs) higher than those (SIs) (P < 0.05). organisms showed similar pattern: superoxide dismutase (SOD) activity glutathione/oxidized glutathione (GSH/GSSG) in cell-free hemolymph hepatopancreases BIs SIs There no significant differences α2-macroglobulin (α2-M), antibacterial bacteriolytic activities hemolymph, or peroxidase hepatopancreas between SIs. α2-M crustin gene expression levels hemocytes, SOD hemocytes hepatopancreas, also significantly BIs. results suggest that, compared SIs, possessed resistance to V. alginolyticus infection.
sci-hub.se 参考文章(51)
Mary E. Anderson, [70] Determination of glutathione and glutathione disulfide in biological samples Glutamate, Glutamine, Glutathione, and Related Compounds. ,vol. 113, pp. 548- 555 ,(1985) , 10.1016/S0076-6879(85)13073-9
P. B. Armstrong, J. P. Quigley, F. R. Rickles, The Limulus Blood Cell Secretes α2-Macroglobulin When Activated. The Biological Bulletin. ,vol. 178, pp. 137- 143 ,(1990) , 10.2307/1541972
L. Góth, A simple method for determination of serum catalase activity and revision of reference range Clinica Chimica Acta. ,vol. 196, pp. 143- 151 ,(1991) , 10.1016/0009-8981(91)90067-M
So Young Lee, Bok Luel Lee, Kenneth Söderhäll, Processing of crayfish hemocyanin subunits into phenoloxidase. Biochemical and Biophysical Research Communications. ,vol. 322, pp. 490- 496 ,(2004) , 10.1016/J.BBRC.2004.07.145
Long Tao Wu, Ka Hou Chu, Characterization of an ovary-specific glutathione peroxidase from the shrimp Metapenaeus ensis and its role in crustacean reproduction. Comparative Biochemistry and Physiology B. ,vol. 155, pp. 26- 33 ,(2010) , 10.1016/J.CBPB.2009.09.005
Xian-liang Meng, Ping Liu, Jian Li, Bao-Quan Gao, Ping Chen, Physiological responses of swimming crab Portunus trituberculatus under cold acclimation: Antioxidant defense and heat shock proteins Aquaculture. ,vol. 434, pp. 11- 17 ,(2014) , 10.1016/J.AQUACULTURE.2014.07.021
Chenglong Ji, Huifeng Wu, Lei Wei, Jianmin Zhao, iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A. Aquatic Toxicology. ,vol. 157, pp. 30- 40 ,(2014) , 10.1016/J.AQUATOX.2014.09.008
Kohsuke Adachi, Takashi Hirata, Takaaki Nishioka, Morihiko Sakaguchi, Hemocyte components in crustaceans convert hemocyanin into a phenoloxidase-like enzyme. Comparative Biochemistry and Physiology B. ,vol. 134, pp. 135- 141 ,(2003) , 10.1016/S1096-4959(02)00220-8
Ya'nan Yang, Chenchang Bao, An Liu, Haihui Ye, Huiyang Huang, Shaojing Li, Immune responses of prophenoloxidase in the mud crab Scylla paramamosain against Vibrio alginolyticus infection: In vivo and in vitro gene silencing evidence Fish & Shellfish Immunology. ,vol. 39, pp. 237- 244 ,(2014) , 10.1016/J.FSI.2014.05.014
Doris Abele, Susana Puntarulo, Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish Comparative Biochemistry and Physiology A-molecular & Integrative Physiology. ,vol. 138, pp. 405- 415 ,(2004) , 10.1016/J.CBPB.2004.05.013