23S rRNA-targeted Oligonucleotide Probes for the Rapid Identification of Meat Lactobacilli

作者: Christian Hertel , Wolfgang Ludwig , Michaela Obst , Rudi F. Vogel , Walter P. Hammes

DOI: 10.1016/S0723-2020(11)80298-5

关键词:

摘要: Summary A method for the rapid identification of Lactobacillus species that are used as starter organisms fermented meat products was developed. Sequences 23S rRNA specific curvatus, L. sake, and pentosus/L. plantarum were identified complementary oligonucleotides synthesized. The specificity probes checked by dot blot colony hybridizations. can be analysis mixed cultures. None other Gram-positive bacteria found in reacted with oligonucleotide probes.

参考文章(17)
Ellson Y. Chen, Peter H. Seeburg, Supercoil sequencing: a fast method for sequencing plasmid DNA DNA. ,vol. 4, pp. 165- 170 ,(1985)
in chief George M. Garrity, Bergey's Manual of Systematic Bacteriology ,(1986)
Michèle Delley, Beat Mollet, Herbert Hottinger, DNA Probe for Lactobacillus delbrueckii. Applied and Environmental Microbiology. ,vol. 56, pp. 1967- 1970 ,(1990) , 10.1128/AEM.56.6.1967-1970.1990
K.R. Schleifer, DNA probes in food microbiology Food Biotechnology. ,vol. 4, pp. 585- 598 ,(1990) , 10.1080/08905439009549773
E. DeLong, G. Wickham, N. Pace, Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells Science. ,vol. 243, pp. 1360- 1363 ,(1989) , 10.1126/SCIENCE.2466341
ELLSON Y. CHEN, PETER H. SEEBURG, Supercoil sequencing: a fast and simple method for sequencing plasmid DNA DNA (Mary Ann Liebert, Inc.). ,vol. 4, pp. 165- 170 ,(1985) , 10.1089/DNA.1985.4.165
David T. Denhardt, A membrane-filter technique for the detection of complementary DNA Biochemical and Biophysical Research Communications. ,vol. 23, pp. 641- 646 ,(1966) , 10.1016/0006-291X(66)90447-5
R. Saiki, D. Gelfand, S Stoffel, S. Scharf, R Higuchi, G. Horn, K. Mullis, H. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science. ,vol. 239, pp. 487- 491 ,(1988) , 10.1126/SCIENCE.2448875
Hermann J. Knauf, Rudi F. Vogel, Walter P. Hammes, Introduction of the transposon Tn919 into Lactobacillus curvatus Lc2-c Fems Microbiology Letters. ,vol. 65, pp. 101- 104 ,(1989) , 10.1111/J.1574-6968.1989.TB03604.X