The Abundance ofZoogloea ramigerain Sewage Treatment Plants

摘要: 19544 T (T 5typestrain)andtwomisclassifiedstrains,Z.ramigeraATCC25935andATCC19623.Dissociation temperatures were determined, and probe specificities, as well the potential of probes for whole-cell hybridization, evaluated by using numerous reference organisms. Several activated sludge samples examined with these both in situ dot blot hybridization methods. Only type strain hybridized to cells that accumulated typical branched gelatinous matrices, so-calledZoogloea fingers.Thisproberevealedcellsinmostoftheactivatedsludgesamplesstudied.Wefoundthatrelativelyhigh levelsofZ.ramigeracells(uptoapproximately10%ofthetotalnumberofcells)andtypicalmorphologytended tobelinkedtooverloadingofsewageplants.TheprobedirectedtorejectedtypestrainZ.ramigeraATCC19623 bound only a few cells. Cells reacted complementary toZ. ramigeraATCC 25935, which was originally isolated from tricklingfilter, not observed sludge. The high levels sensitivity specificity rRNA-targeted nucleic acid make them excellent tools determinative, phylogenetic, environmental studies microbiology (3). Hybridization such is an increasingly accepted method monitoring populations microorganisms, especially microorganisms are difficult cultivate (for reviews see references 1 2). It special attribute fluorescently labeled versions can be readily used hybridization(3,7).Thisuseisfacilitatedbythenaturalamplificationof