Abnormal intrinsic esophageal innervation in congenital diaphragmatic hernia: a likely cause of motor dysfunction.

摘要: Abstract Purpose Patients with congenital diaphragmatic hernia (CDH) often have dilated esophagus and gastroesophageal reflux. Sparse intrinsic esophageal innervation has been described in rats CDH, but this issue not investigated patients CDH. The present study tests the hypothesis that innervatory anomalies could account for motor dysfunction human Methods esophagi of CDH (n = 6) control babies dead other causes were included paraffin, transversally sectioned, immunostained antineurofilament anti–S-100 antibodies. proportion section surface occupied by neural structures, ganglionar surface, number neurons per ganglion measured 2 to 5 low-power fields from proximal distal assistance image analysis software. Mann-Whitney used comparing results using a threshold significance P Results percentage neural/muscle was similar upper both groups, it significantly decreased lower comparison controls. There relative scarcity tissue intermuscular plexus esophagus. On hand, identical groups. These immunostainings. Conclusion Intrinsic is abnormal terms density structures plexus. crest-derived explain part survivors