Development of a rubella virus DNA vaccine

摘要: Two rubella virus DNA vaccines were constructed from a cDNA clone of the genomic RNA, one which contained coding sequences for all three virion proteins (C, E2 and E1) two envelope glycoproteins (E2 E1). When used to immunize mice via gene gun delivery, both constructs induced an antibody response equivalent titer that by persisted at least seven months. A booster injection given four weeks after initial increased titers over thirty-fold. The in vaccine-injected was directed primarily against E1 glycoprotein, as case virus-injected mice, neutralizing activity detected. These are thus prototypes nonreplicating vaccine could be specialized circumstances.