The neutrophil, not the tumor

摘要: BACKGROUND Patients with resected breast carcinoma who received granulocyte—colony-stimulating factor (G-CSF)–supported adjuvant chemotherapy exhibited an increase in their serum CA 15-3 levels. The authors investigated the role of G-CSF–induced neutrophil MUC1 expression this setting. METHODS Twenty-two patients early and 6 high-grade lymphoma cycles or without G-CSF support. When given, was administered for either 5 10 days per cycle. Immunocytochemical staining flow cytometric analysis peripheral blood neutrophils bone marrow myeloid cells epitopes were performed during treatment. RESULTS At baseline, median 16 U/mL, weak (median immunocytochemical score [ICCS] = 40, [FCS] 211 antibody molecules neutrophil). For receiving 5-day support, levels increased moderately 28 U/mL; P 0.016) absolute counts (ANC) did not increase, whereas ICC showed a moderate ICCS 105; 0.015). 10-day G-CSF, 2–4-fold from baseline reached abnormal 47; < 0.0005) ANC 21,400/mm3; 0.007), significant induction occurred cell membrane mostly cytoplasm 162; 0.001). Flow cytometry detected cytoplasmic, but surface, group only (baseline FCS 3975, 4th cycle 6327 cell; 0.028). In marrow, observed band forms neutrophils, more immature cells. Serum found to demonstrate linear relation. incorporated combined score, its relation demonstrated parabolic (cubic) pattern. levels, ANC, returned after completion therapy. No excess malignant recurrences observed. CONCLUSIONS Women G-CSF–primed elevation due number induced cytoplasmic expression, which caused by G-CSF. Physicians should be aware interaction. Cancer 2004. © 2004 American Society.