Polygonum multiflorum Thunb suppress bile acid synthesis by activating Fxr-Fgf15 signaling in the intestine.

摘要: Abstract Ethnopharmacological relevance Polygonum multiflorum Thunb (Heshouwu, HSW) is commonly used in clinical medicine, while the hepatotoxicities of HSW are reported increasingly recent years. Currently, researchers have demonstrated an essential role Bile Acids (BAs) liver diseases. The occurrence hepatotoxicity cases linked to characterized by jaundice and cholestasis, suggesting interaction that between BAs Aim study This was designed investigate HSW-induced functional histological changes mice on bile acid synthesis, metabolism, clearance intestinal absorption. Materials methods were intragastrically (i.g.) given at doses 1.275 and 3.825 g/kg (Crude extracts /body weight) once a day for seven days. Liver function evaluated measuring serum levels enzymes analyzing histology. LC/MS analysis performed quantify from liver, ileum serum. Moreover, expression metabolic-related transporters metabolic both protein mRNA observed elucidate underlying mechanisms. Results Oral administration 7 days could not cause damage. A significant change concentrations treatment groups compared with normal control. excretory transporter (Bsep) basolateral uptake (Ntcp) increased development HSW. unconjugated intestines after Western blot qRT-PCR analyses showed upregulated Shp Fgf15 mice. Conclusion 7days did accelerated enterohepatic circulation changed composition BAs, leding activation Fxr-Fgf15 signal intestines, further inhibited Cyp7a1 liver.