Selecting ligand agonists and antagonists
作者： Mark Ultsch , James A. Wells , Brian C. Cunningham , Abraham M. DeVos , Michael G. Mulkerrin
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摘要: We have discovered that growth hormones form ternary complexes with their receptors in which site 1 on the hormone first binds to one molecule of receptor and then 2 another receptor, thereby producing a 1:2 complex. believe this phenomenon is shared by other ligands having similar conformational structure. Assays based are useful for identifying ligand agonists antagonists. Sites structurally identified facilitate generation amino acid sequence variants complex-forming ligands. Novel hormone, prolactin placental lactogen related provided. As result our studies complex we determined selected antibodies these capable acting as or novel uses anti-growth described.
lens.org参考文章(68)
Jack L. Kostyo, The Multivalent Nature of Growth Hormone Springer, Boston, MA. pp. 449- 453 ,(1986) , 10.1007/978-1-4615-7201-5_35
R Barnard, P G Bundesen, D B Rylatt, M J Waters, Evidence From the Use of Monoclonal-Antibody Probes for Structural Heterogeneity of the Growth-Hormone Receptor Biochemical Journal. ,vol. 231, pp. 459- 468 ,(1985) , 10.1042/BJ2310459
Alvin Leo Melliere, David Bennett Anderson, Darrell Jay Jones, Phenethanolamine and growth hormone combinations ,(1989)
R. Devos, G. Plaetinck, J. Van der Heyden, S. Cornelis, J. Vandekerckhove, W. Fiers, J. Tavernier, Molecular basis of a high affinity murine interleukin-5 receptor. The EMBO Journal. ,vol. 10, pp. 2133- 2137 ,(1991) , 10.1002/J.1460-2075.1991.TB07747.X
Lennart Olsson, Composite binding site drugs ,(1989)
Menachem Zeevi, Marian Gorecki, Tikva Vogel, Haim Aviv, Avigdor Levanon, Amos Oppenheim, Pinhas E. Zeelon, Analogs of hGH having antagonistic activity, and uses thereof ,(1985)
J. C. Porter, J. F. Sissom, M. L. Eigenbrodt, Anti-growth action on mouse mammary and prostate glands of a monoclonal antibody to prolactin receptor American Journal of Pathology. ,vol. 133, pp. 589- 595 ,(1988)
Shmaryahu Blumberg, Meir Daniela Ben, Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby ,(1985)
C H Heldin, A Ernlund, C Rorsman, L Rönnstrand, Dimerization of B-type platelet-derived growth factor receptors occurs after ligand binding and is closely associated with receptor kinase activation. Journal of Biological Chemistry. ,vol. 264, pp. 8905- 8912 ,(1989) , 10.1016/S0021-9258(18)81879-8
N.A. Lokker, N.R. Movva, U. Strittmatter, B. Fagg, G. Zenke, Structure-activity relationship study of human interleukin-3. Identification of residues required for biological activity by site-directed mutagenesis. Journal of Biological Chemistry. ,vol. 266, pp. 10624- 10631 ,(1991) , 10.1016/S0021-9258(18)99269-0