Preparation method for the production of active and soluble proteins in prokaryotes and polycistronic vectors therefor

作者: Oh Byung Poongsan Haetvitmaeul Apt Kwon , Tai Young Koo , Bong Yong Lee , Song Young Kim , Kyung Hyun Min

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摘要: Disclosed are a method of producing target protein in biologically-active, soluble form prokaryotic cells and polycistronic vectors therefor.

参考文章(24)
Akiko Togi, Masahiro Furutani, Junichi Hata, Process for production of a recombinant protein and a fusion protein ,(2007)
F. William Studier, Alan H. Rosenberg, John J. Dunn, John W. Dubendorff, Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology. ,vol. 185, pp. 60- 89 ,(1990) , 10.1016/0076-6879(90)85008-C
Daniel G. Yansura, Dorothea Reilly, Eriks Sasha Paegle, Process for production of polypeptides ,(2002)
Thomas E. Creighton, André Zapun, Nigel J. Darby, Mechanisms and catalysts of disulphide bond formation in proteins Trends in Biotechnology. ,vol. 13, pp. 18- 23 ,(1995) , 10.1016/S0167-7799(00)88896-4
Max E Gottesman, Wayne A Hendrickson, Protein folding and unfolding by Escherichia coli chaperones and chaperonins Current Opinion in Microbiology. ,vol. 3, pp. 197- 202 ,(2000) , 10.1016/S1369-5274(00)00075-8
Björn Nilsson, Tomas Moks, Birger Jansson, Lars Abrahmsén, Anette Elmblad, Erik Holmgren, Christina Henrichson, T.Alwyn Jones, Mathias Uhlén, A synthetic IgG-binding domain based on staphylococcal protein A Protein Engineering. ,vol. 1, pp. 107- 113 ,(1987) , 10.1093/PROTEIN/1.2.107
Andrew D. Guise, Shuna M. West, Julian B. Chaudhuri, Protein folding in vivo and renaturation of recombinant proteins from inclusion bodies. Molecular Biotechnology. ,vol. 6, pp. 53- 64 ,(1996) , 10.1007/BF02762323
J.T. Moore, A. Uppal, F. Maley, G.F. Maley, Overcoming inclusion body formation in a high-level expression system. Protein Expression and Purification. ,vol. 4, pp. 160- 163 ,(1993) , 10.1006/PREP.1993.1022