DNA-induced inter-particle cross-linking during expanded bed adsorption chromatography. Impact on future support design.

摘要: We have investigated the effects of adsorbent size, ionic capacity and surface immobilised polymers on dynamic changes occurring to beds anion-exchangers during binding DNA. During application low concentrations “3–20 kilobase” calf thymus DNA feeds expanded anion-exchangers, bed heights dropped progressively as molecules physically cross-linked neighbouring particles together, form severely aggregated fluidised beds. In plots capacities absolute in porosity at maximum contraction, against inverse mean hydrated particle radii, were observed split into three distinct, but different clusters each case. The highest index packing was for two prototype pellicular supports, one derivatised with highly charged high molecular mass polyethyleneimine (Mr∼50 000) other long dextran (Mr∼500 chains weakly DEAE. However, ability surfaces these matrices bring about strikingly different. afforded by coupling exhibited a three-fold higher tendency interact presence than that DEAE support. implications findings design future materials separation both proteins nucleic acids are discussed.